antibodies-specific for ep1, ep2, ep3, and ep4  (Cayman Chemical)

Journal: Scientific Reports

Article Title: PGE 2 maintains self-renewal of human adult stem cells via EP2-mediated autocrine signaling and its production is regulated by cell-to-cell contact

doi: 10.1038/srep26298

Figure Lengend Snippet: ( a ) EP receptor expression in hUCB-MSCs and hAD-MSCs was assessed by Western blot analysis. HMO6, a human microglia cell line, was used as a positive control. In the presence of selective blockers for EPs, ( b ) cell growth rates were examined by BrdU assay and ( c ) results of repeated experiments are presented. Selective blockers for EP1: SC-51089, EP2: AH-6809, EP3: L-798106, and EP4: L-161,982. ( d ) After celecoxib treatment, cells were treated with selective agonists for EP2 or EP3, and proliferation was measured with a BrdU assay kit. Gel electrophoresis was conducted under the same experimental conditions, and images of blots were cropped. Selective agonist for EP2: Butaprost, EP3: Sulprostone. * P < 0.05, ** P < 0.01. Results are shown as the mean ± SEM.

Article Snippet: After blocking with 3% bovine serum albumin (BSA) solution, proteins on the membrane were incubated with primary antibodies against COX-2, mPGES-1 (Abcam, Cambridge, MA, USA), EP1, EP2, EP3, EP4 (Cayman, Ann Arbor, MI, USA), iNOS and IDO-1 (Millipore, Billerica, MI, USA) more than 12 hours at 4 °C and then incubated with secondary antibodies.

Techniques: Expressing, Western Blot, Positive Control, BrdU Staining, Nucleic Acid Electrophoresis

Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Concurrent targeting of EP1/EP4 receptors and COX-2 induces synergistic apoptosis in KSHV and EBV associated non-Hodgkin lymphoma cell lines

doi: 10.1016/j.trsl.2013.02.008

Figure Lengend Snippet: (a) Total lysates from 5×105 BJAB, Akata/EBV−, BCBL-1, BC-3, Akata/EBV+, and JSC-1 cells were immunoblotted for EP1, EP2, EP3, and EP4. Data represents three independent experiments. Tubulin was used as loading control. (b) In parallel experiments, supernatants from the indicated cells were collected to measure the amount of PGE2 secreted by each cell line. (c) The indicated cell lines were stained for EP1, EP2, EP3, and EP4 receptors by immunofluorescence. (d) Mean fluorescent intensity (MFI) of EP1, EP2, EP3, and EP4 receptors in HMVEC-d cells infected with KSHV measured by FACS. Indicated are the MFI for the respective receptor at each time point. The data is representative of three independent experiments.

Article Snippet: Mouse monoclonal antibodies against human COX-2 and rabbit polyclonal antibodies against EP1, EP2, EP3, and EP4 receptors were purchased from Cayman Chemicals, Ann Arbor, MI.

Techniques: Control, Staining, Immunofluorescence, Infection

Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Concurrent targeting of EP1/EP4 receptors and COX-2 induces synergistic apoptosis in KSHV and EBV associated non-Hodgkin lymphoma cell lines

doi: 10.1016/j.trsl.2013.02.008

Figure Lengend Snippet: Effect of concurrent targeting of COX-2 and EP1/EP4 receptors with 1.0µM each of celecoxib, SC-51322, and GW 627368X on various classes of NHL genes

Article Snippet: SC-51322 (EP1 antagonist) was purchased from Enzo Life Sciences, Plymouth Meeting, PA. Antibodies Mouse monoclonal antibodies against human COX-2 and rabbit polyclonal antibodies against EP1, EP2, EP3, and EP4 receptors were purchased from Cayman Chemicals, Ann Arbor, MI.

Techniques:

Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Concurrent targeting of EP1/EP4 receptors and COX-2 induces synergistic apoptosis in KSHV and EBV associated non-Hodgkin lymphoma cell lines

doi: 10.1016/j.trsl.2013.02.008

Figure Lengend Snippet: (a) Total lysates from 5×105 BJAB, Akata/EBV−, BCBL-1, BC-3, Akata/EBV+, and JSC-1 cells were immunoblotted for EP1, EP2, EP3, and EP4. Data represents three independent experiments. Tubulin was used as loading control. (b) In parallel experiments, supernatants from the indicated cells were collected to measure the amount of PGE2 secreted by each cell line. (c) The indicated cell lines were stained for EP1, EP2, EP3, and EP4 receptors by immunofluorescence. (d) Mean fluorescent intensity (MFI) of EP1, EP2, EP3, and EP4 receptors in HMVEC-d cells infected with KSHV measured by FACS. Indicated are the MFI for the respective receptor at each time point. The data is representative of three independent experiments.

Article Snippet: SC-51322 (EP1 antagonist) was purchased from Enzo Life Sciences, Plymouth Meeting, PA. Antibodies Mouse monoclonal antibodies against human COX-2 and rabbit polyclonal antibodies against EP1, EP2, EP3, and EP4 receptors were purchased from Cayman Chemicals, Ann Arbor, MI.

Techniques: Staining, Immunofluorescence, Infection

Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Concurrent targeting of EP1/EP4 receptors and COX-2 induces synergistic apoptosis in KSHV and EBV associated non-Hodgkin lymphoma cell lines

doi: 10.1016/j.trsl.2013.02.008

Figure Lengend Snippet: Effect of concurrent targeting of COX-2 and EP1/EP4 receptors with 1.0µM each of celecoxib, SC-51322. and GW 627368X on various classes of NHL genes.

Article Snippet: SC-51322 (EP1 antagonist) was purchased from Enzo Life Sciences, Plymouth Meeting, PA. Antibodies Mouse monoclonal antibodies against human COX-2 and rabbit polyclonal antibodies against EP1, EP2, EP3, and EP4 receptors were purchased from Cayman Chemicals, Ann Arbor, MI.

Techniques: